Study on the mechanism of Chinese medicine compound inhibiting proliferation of leukemia cells

Blood, one of the top magazines in the blood field. Although it was published ten years ago, it seems to be very useful.

The disease is leukemia , and the drug is a traditional Chinese medicine compound Huanglian Jiedu Decoction (HLJDT), a medicinal material of Astragalus membranaceus and an extracted monomeric baicalein .

Before the official sharing, let's talk about other things. After the introduction of leukemia at the beginning of this article ("Clinical Performance" in the National Nature Lecture), the introduction of the Chinese medicine section, in addition to introducing curcumin, arsenic flowers , also highlighted Artemisinin. It seems that the work of the grandmother has long been famous in the industry.

Unfortunately, the word used in the article to extract Huanglian Jiedu Tang is Kampo medicine, not Traditional Chinese medicine. Kampo, Han Fang, is the name of Japan. I don't know if the trade name and patent of Huanglian Jiedu Decoction is Chinese or Japanese. We will not work hard anymore. The future name is "Hanfang" or "Korean Medicine".

Figure 1. Effect of HLJDT, GZFLW, or HLT on proliferation of myeloma cell lines.

In addition to HLJDT, the three prescriptions are HLT, and GZFLW is a prescription that does not inhibit immunity.

Conclusion HLJDT can inhibit the proliferation of leukemia cells.

Figure 2. Effect of HLJDT, GZFLW, or HLT on proliferation of primary myeloma cells.

The drug effect was again detected by primary isolated leukemia cells. HLJDT can inhibit MPC-leukemia cells.

Figure 3. HLJDT induced apoptosis in U266 cells.

The number of viable cells was detected in the previous section. This time, the detection method was used to see the apoptosis. It is concluded that HLJDT can induce leukemia cell apoptosis.

Figure 4. Identification of baicalein as the active component of Scutellaria radix (root) in HLJDT.

The first three figures have shown that the prescription is effective. Worried that the high score SCI reviewer will ask the effective ingredients is awkward, the author began to explore the effective ingredients. Haha, Ji Bo smashed. The more research is now, the better. The author considers it from the perspective of scientific research. First explored the medicinal material of Astragalus membranaceus, and found that the root of Astragalus membranaceus also has a killing effect. After removing the root of Astragalus membranaceus in HLJDT, the killing effect is reduced (Figure A). Everyone noticed that there is more than one effective medicinal material here. This article only explores the roots of Astragalus.

Then explore the ingredients inside the root of the yellow scorpion , do three, baicalein , baicalin, wogonin. All have a killing effect, the best is baicalein . I believe that there are more than three effective ingredients. This article only discusses these three. Compared to the compound, the single medicinal material has achieved a clear level of composition.

Figure 5. Flow cytometric histogram of cells cultured with baicalein.

The primary cells of the patient were used to detect the killing effect of baicalein.

Figure 6. Effect of baicalein, baicalin, or wogonin on the survival of primary myeloma cells in vitro.

The primary cells of the patient are still used to detect the killing effect of these three monomers.

Figure 7. Effect of baicalein on phosphorylation of IkB-α, expression of NF-kB target genes, and mitochondrial apoptotic pathway.

How can the drug research on the tall side lack the molecular mechanism? In the last picture, the authors examined the molecular mechanisms downstream of the drug, all known molecules.

to sum up

1. Validation of the compound validity. (Cell function, the original strain was used on the cell line, instead of the animal experiment.)

2. Effective medicinal materials in the compound. There is usually no more effective medicinal material, but I only do one in a study, unless it is to explore the relationship between the two (the same as the cell model is to put one class or put more classes. For details, please refer to "National Nature" Three-cell model and detection indicators.)

3. The active ingredients of effective medicinal materials. By the same token, there will be only one active ingredient. In this study, I only focus on one.

4. The molecular mechanism of the active ingredients. For new drugs, classic downstream molecules (old genes) are generally used.

This article is a classic article on the study of traditional Chinese medicine prescriptions, from the discussion of the effectiveness of prescriptions to the discussion of effective medicinal materials, to the discussion of active ingredients, and finally to the mechanism of active ingredients. The research in this paper brings us to the clinical use of monomeric baicalein instead of HLJDT. It is a preliminary exploration of why the complex component of HLJDT can play a role in leukemia. Solve the shortcomings of the traditional Chinese medicine compound "who is the active ingredient and what is the mechanism". The effective ingredients and mechanisms of HLJDT are definitely more than just these. What we have to do is to continue to explore.

If you are doing oncology research, you can look at other effective ingredients in HLJDT or Astragalus root when you continue to explore, or you can continue to explore the mechanism of baicalein killing tumor cells, looking for new downstream functional genes ( old Medicine, new genes, new relationship ). On the basis of this article, your continued exploration, as long as it is deep enough, sends a higher article.

PS: At the beginning of this article, I wanted to explore how HLJDT inhibits leukemia by immunization, because the prescription is theoretically immune-related. In actual experiments, there is no IL6, and the results are also different. However, the article did not continue to explore this direction in the end, which is the flaw of this article.

In the next article, we share the 2012 article, which is also from the compound, is an old drug, and the molecular mechanism is deep. Stay tuned.

EVERYBODY: I hope that friends who have more exchanges with Jibo in learning and experimentation can pay attention to Jikai Gene WeChat and reply to the words “Jibo”.

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