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Friends who have worked in the laboratory for a long time have the feeling that the cleaning of laboratory glassware has become an important task in the daily work of the laboratory. Many friends encounter various problems during the cleaning process. Today, I mainly talk about glassware cleaning in laboratories such as pharmaceuticals, biology, and chemistry.
Glassware commonly used in laboratories: volumetric flasks, conical flasks, beakers, pipettes, flasks, LC and GC vials, etc.
First, the following describes the cleaning agents commonly used in the laboratory:
1, chromic acid washing solution
Chromium is carcinogenic, so be careful when formulating and using lotions. Two common methods are as follows:
(1) Take 100 mL of industrial concentrated sulfuric acid in a beaker, carefully heat, then slowly add 5 g of potassium dichromate powder, stir while stirring, and after all dissolved and slowly cooled, store in a fine-mouthed bottle of a ground glass stopper.
(2) Weigh 5g potassium dichromate powder, place it in a 250mL beaker, add 5mL water to dissolve it, then slowly add 100mL concentrated sulfuric acid, the solution temperature will reach 80 °C, wait for it to cool and store it in the ground glass bottle.
2, industrial concentrated hydrochloric acid: can be washed off scale or some inorganic salts precipitated.
3, 5% oxalic acid solution: acidified with a few drops of sulfuric acid, can wash away traces of potassium permanganate.
4, 5% ~ 10% trisodium phosphate (Na3PO4 · 12H2O) solution: can wash oil stains.
5, 30% nitric acid solution: washing carbon dioxide analyzer and micro dropper.
6. 5% to 10% disodium edetate (EDTA-Na2) solution: heating and boiling can elute the white precipitate on the inner wall of the glass instrument.
7. Urea washing solution: a good solvent for protein, suitable for washing containers containing protein preparations and blood samples.
8, organic solvents: such as acetone, ether, ethanol, etc. can be used to elute oil, fat-soluble dye stains, etc., xylene can elute paint dirt.
9. Ethanol solution of potassium hydroxide and sodium hydroxide solution containing potassium permanganate: These are two kinds of strong alkaline washing liquids, which are highly corrosive to glass instruments, can remove dirt on the inner wall of the container, and the washing time should not be excessive. Long, should be used with caution (this lotion is also commonly used to clean LC, GC injection bottles).
10. In the laboratory, the cleaning agent is purchased in the world. It is the most professional cleaning agent in the world: Swiss Borer. The company's products are phosphate-free, divided into manual cleaning and machine washing. It is recommended to use a wave cleaner for the bottle washer.
Second, introduce the way of cleaning glassware commonly used in several laboratories
The degree of automation is as follows:
1, manual cleaning
2, automatic laboratory bottle washer cleaning
3, manual immersion + ultrasonic cleaning
From the cleaning process, it is as follows:
1. The general glass instrument is first rinsed with tap water, then scrubbed with a conventional lotion, then washed with tap water, and finally rinsed three times with water;
2, precision or difficult to brush utensils (pipettes, volumetric flasks, conical flasks, etc.) first rinse with tap water, drain, soaked with washing liquid, then rinse with tap water, and finally rinse with pure water;
3, a cleaned glassware should be free of oil, no water drops, otherwise it should be washed again until the requirements are met.
4, automatic bottle washer cleaning: For example, the standard cleaning of glassware is as follows: pre-wash cold water cleaning, 60 ° C water temperature alkaline cleaning agent cleaning, neutral cleaning with acidic cleaning agent, soft water cleaning, pure water cleaning, pure water rinsing (up to 95 ° C). The cleaning is all over.
5, washing machine cleaning procedures are very important, please be sure to follow the manufacturer's reference procedures, water temperature, water circulation, the amount of cleaning agent, the number of rinses on the vessel's clean index are very high requirements.
Third, briefly describe the current pattern of laboratory washing machines in China:
At present, the domestic bottle washing machine is still in its infancy compared with foreign countries. However, the domestic Duobate brand bottle washing machine and Ningbo Xinzhi automatic washing machine are relatively mature in China, and Ningbo Xinzhi is more mature in the domestic medical field. Outstanding, the language bottle is more concerned with high-end laboratories, such as pharmaceutical companies, petrochemical refining companies. It is also hoped that the domestic bottle washing machine can develop faster and get more laboratory approval.
To sum up: At present, most domestic laboratories use manual cleaning, but the residue verification, cleaning effect and cleaning verification are not better reflected. The author still hopes that the conditional laboratory will focus on GC-MS, LC- Laboratory for trace detection such as MS, recommended laboratory bottle washer.