I don't know if anyone who works in the lab has encountered this situation: I wanted to remove a sample of the solution, but found that the pipette seemed to be inaccurate and needed to be calibrated. First, we need to prepare an electronic balance that has been calibrated, then put a small beaker and peel it (ie remove the weight of the container such as the basket containing the item). Pipette a certain amount of distilled water (the amount of distilled water here requires us to remember how much it is removed) and then weigh it into a small beaker for weighing. The mass divided by the density is the actual volume of distilled water. Of course, it is best to measure the water temperature before we remove the distilled water, because the density of distilled water will be different at different temperatures. The density of distilled water at different temperatures is shown in the table below. Density of distilled water at different temperatures temperature 0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 Difference X10 5 0.9999919 0.9999902 0.9999883 0.9999842 0.9999842 0.9999819 0.9999795 0.9999769 0.9999741 0.9999712 1 6 0.9999681 0.9999649 0.9999616 0.9999581 0.9999544 0.9999506 0.9999467 0.9999426 0.9999384 0.999934 1 7 0.9999295 0.9999248 0.99992 0.999915 0.9999099 0.9999046 0.9998992 0.9998936 0.9998879 0.9998821 2 8 0.9998762 0.9998701 0.9998638 0.9998574 0.9998509 0.9998442 0.9998374 0.9998305 0.9998234 0.9998162 3 9 0.9998088 0.9998013 0.9997936 0.9997859 0.999778 0.9997699 0.9997617 0.9997534 0.999745 0.9997364 5 10 0.9997277 0.9997189 0.9997099 0.9997008 0.9996915 0.999682 0.9996724 0.9996627 0.9996529 0.9996428 6 11 0.9996328 0.9996225 0.9996121 0.9996017 0.9995911 0.9995803 0.9995694 0.9995585 0.9995473 0.9995361 2 12 0.9995247 0.9995132 0.9995016 0.9994898 0.999478 0.999466 0.9994538 0.9994415 0.9994291 0.9994166 0 13 0.999404 0.9993913 0.9993784 0.9993655 0.9993524 0.9993391 0.9993258 0.9993123 0.9992987 0.999285 1 14 0.9992712 0.9992572 0.9992432 0.999229 0.9992147 0.9992003 0.9991858 0.9991711 0.9991564 0.9991415 1 15 0.9991265 0.9991113 0.9990961 0.9990608 0.9990653 0.9990497 0.999034 0.9990182 0.9990023 0.9989862 2 16 0.9989701 0.9989538 0.9989374 0.9989209 0.9989043 0.9988876 0.9988707 0.9988538 0.9988367 0.9988175 6 17 0.9988022 0.9987849 0.9987673 0.9987497 0.9987319 0.9987141 0.9986961 0.9986781 0.9986599 0.9986416 9 18 0.9986232 0.9986046 0.9985861 0.9985673 0.9985485 0.9985295 0.9985105 0.9984913 0.998472 0.9984326 14 19 0.9984331 0.9984136 0.9983938 0.998374 0.9983541 0.9983341 0.998314 0.9982937 0.9982733 0.9982529 18 20 0.9982323 0.9982117 0.9981909 0.9981701 0.998149 0.998128 0.9981068 0.9981695 0.9980641 0.9980426 twenty two twenty one 0.998021 0.9979993 0.9979775 0.9979556 0.9979335 0.9979114 0.9978892 0.9978869 0.9978444 0.9973219 25 twenty two 0.9977993 0.9977765 0.9977537 0.9977308 0.9977077 0.9976846 0.9976613 0.997638 0.9976145 0.9975918 27 twenty three 0.9975674 0.9975437 0.9975198 0.9974959 0.9974718 0.9974477 0.9974435 0.9973991 0.9973717 0.9973502 29 twenty four 0.9973256 0.9973009 0.997276 0.9972511 0.9972261 0.997201 0.9971758 0.9971505 0.997125 0.9970995 30 25 0.9970739 0.9970432 0.9970225 0.9969966 0.9969706 0.9969445 0.9969184 0.9968921 0.9963657 0.9968393 31 26 0.9968128 0.9967861 0.9967594 0.9967326 0.9967057 0.9966726 0.9966515 0.9966243 0.996597 0.9965696 30 27 0.9965241 0.9965146 0.9964869 0.9964591 0.9964313 0.9964033 0.9963753 0.9963472 0.996319 0.9962907 29 28 0.9962623 0.9962338 0.9962052 0.9961766 0.9961478 0.996119 0.9961901 0.996061 0.9960319 0.9960027 28 29 0.9959735 0.995944 0.9959146 0.995885 0.9958554 0.9950257 0.9957958 0.9957659 0.9957359 0.9957059 25 30 0.9956756 0.9956454 0.9956151 0.9955846 0.9955541 0.9955235 0.9954928 0.995462 0.9954312 0.9954002 twenty three 31 0.9953692 0.995338 0.9953068 0.9952755 0.9952442 0.9952127 0.9951812 0.9951495 0.9951178 0.9950861 20 32 0.9950542 0.9950222 0.9949901 0.994958 0.9959258 0.9948935 0.9948612 0.9948286 0.9947961 0.9947635 19 33 0.9947308 0.994698 0.9946651 0.9946321 0.9945991 0.994566 0.9945328 0.9944995 0.9944661 0.9944327 17 34 0.9943991 0.9943655 0.9943319 0.9942981 0.9942643 0.9942303 0.9941963 0.9941622 0.994128 0.9940938 16 35 0.9940594 0.9940251 0.9949906 0.994956 0.9949214 0.9948867 0.9948518 0.994817 0.994782 0.994747 16 Seafood Mix,Cooked Mussel Meat,Mussel Half Shell,Cut Swimming Crab GOLD STAR FISHERY ZHOUSHAN CO.,LTD. , https://www.goldstar-aquatic.com
I don't know how everyone is doing this kind of situation, but if someone like me is not particularly demanding and afraid of trouble, and I don't want to go to the calibration unit, I can use the method I will talk about below, that is. Use an electronic balance to calibrate the pipette. So what do we do specifically?