Pipette tip - small tip, university ask

The pipette tip, like the bullet of a gun, is an indispensable accessory for pipettes. In theory, the quality of the tip has a great influence on the accuracy of the pipetting, and its importance is no less than that of the pipette itself.

Let me talk about the material of the pipette tip.

The tips on the current pipette tip market are basically made of polypropylene plastic (a colorless transparent plastic with a high chemical inertness and a wide temperature range). However, the same polypropylene, there will be a big difference in quality: high-quality tips are generally made of natural polypropylene, and cheap tips are likely to use recycled polypropylene plastic (in this case) , we can only say that its main component is polypropylene). In addition, most of the tips will be added with a small amount of additives during the manufacturing process. The more common additives are: 1. Color-developing materials. The commonly known blue tip (1000ul) and yellow tip (200ul) on the market are the corresponding color-developing materials added to polypropylene. (As a user, it is definitely desirable to have high-quality masterbatch instead of inexpensive industrial pigments. ); 2, release agent. Mainly used to help the tip quickly detach from the mold after forming. In this case, the more additives, the higher the probability that an undesired chemical reaction may occur during pipetting. So the safest is to add no additives. However, due to the relatively high requirements on the production process, pipette tips with no additives at all are really rare on the market, so our users can only choose pipette tips with less additives when choosing.

Followed by the packaging of the pipette tip.

The pipette tips are mainly packaged in bags and boxes. In the relatively mature market, the boxed majority; in the Chinese market, the bag is currently the absolute mainstream - the main reason is that the bag is cheap. The so-called bag is to put the tip in a plastic ziplock bag, 500 or 1000 per bag (the number of bags per lot will be much less). Most users will manually put the tip into the tip box after buying the bag tip, which in turn increases the chance of contaminating the tip! In addition, in recent years, there has also been a new type of tip packaging in which refills (8-plate or 10-plate tips are stacked in a tower shape, and the tip can be quickly loaded into the tip box without touching the tip). This type of tip requires less storage space and reduces the use of plastic to achieve environmental protection. At present, the share of this type of tip packaging is still very small in our country.

Finally, the price of the pipette tip.

Let's first talk about bagged tips (based on 10ul, 200ul and 1000ul, 1000 bags per bag). The bag tip is mainly divided into three grades: one, the imported tip. According to Lebe, the most expensive is Eppendorf, a bag of 400-500 yuan; Second, imported brands, domestic production (also known as "fake foreign devils"). The representative brand of this grade is Axygen, its price is generally 60-80 yuan, the market share of Axygen tips is quite high; Third, domestic tips, but domestic manufacturers are generally small in scale, production environment Poor, product control is not strict or not at all. The price range of domestic tips is generally 30-60 yuan. The price of a typical boxed tip is 1.5-2.5 times that of a bagged tip, while the refill tip is 10-20% cheaper than a boxed tip.

In the previous article, Lebe and everyone discussed the material, packaging and price of the pipette tip. In fact, there are some things that our users don't mention but are also important: special tips and tips. Matching, bio-contamination of the tips and filter tips.

the first,   Special tips.

Special tips are specially designed for special samples or special applications. There are mainly the following types: The first type is a gel tip (also called a spotting tip): the tip of the tip is flat or very thin, mainly used for electrophoresis experiments; the second type is a wide mouth tip: sucking The tip of the tip is larger than the general tip, and is mainly used to remove macromolecular solutions such as proteins; the third type is low-adsorption tips: the inner wall of such tips is specially treated to reduce the surface adsorption force and can be used to remove the viscosity. Liquid; the fourth type is the elongated tip: mainly used for taking liquid in containers such as volumetric flasks; the fifth type is external piston type tips: mainly used for external piston pipettes and continuous distributors. On the pipette.

Second, the adaptability of the pipette tip.

The adaptability of pipette tips is a growing concern for users today. Why? Because not all of the tips can be used on pipettes of any brand of the corresponding range, Lebe once again reminds us that the user must pay attention to the fit of the tip when purchasing the tip. Mainly we can understand the adaptability of the tip from the following aspects: First, the specificity of the tip. Some brands of pipettes in some series can only use its own standard tip, and other tips cannot be used. For example, Rainin's multichannel pipette must use its own LTS tip; second, the extent to which the tip fits. The most common case is that a pipette can use a variety of tips, but the effect of pipetting after loading different tips is different. In general, the standard tips are still the best, but some brands are also good, such as Axygen's tips. Third, the tip and pipette range should be adapted. In general, the volume of the tip should be greater than or equal to the maximum range of the pipette. For example, a 200ul tip can be used for pipettes with a maximum range of 20ul, 100ul and 200ul. Fourth, a large-scale pipette (5ml, 10ml and 20ml) speciality of the tip. Large pipettes of major pipette brands often require their standard tips, making it difficult to find alternatives. Therefore, if we need to buy a tip, we recommend telling the supplier the model of the tip we are using or at least the pipette brand and range we use.

third,   Biological pollution.

Nowadays, more and more users will ask for no RNase, no DNase, no DNA, no pyrogen and ATP contamination. This is a good phenomenon, but Lebe has to remind two more points: First, the high temperature sterilized tips are not completely guaranteed to be free of biological contamination, and to ensure that there is no biological contamination, gamma ray processing is required. Therefore, it is almost impossible for the user to treat the ordinary tip into a bio-contaminated tip, and the purchased bio-contaminated boxed tip should provide a corresponding certificate indicating the test data of the corresponding item. There is no data on the certificate or even a tip without a certificate. It cannot be proved to be completely non-polluting. Second, the bagged tip is even less guaranteed to be free of biological contamination. As long as the tip touches the air, there is a possibility of being contaminated by the organism, and the ordinary ziplock bag cannot guarantee that the tip does not touch the air at all during storage and transportation; in addition, the zipper bag is punctured by the tip. occur. Therefore, if your requirements for the tip are relatively high, be sure to check with the supplier if the tip has a certificate indicating the test data of the corresponding item.

Fourth, with a filter tip.

With a filter tip, there is a filter element (usually white) at the top of the tip. This filter element is typically made of polypropylene, similar to the filter construction of cigarettes. Due to the presence of this filter element, the removed sample cannot enter the interior of the pipette, thereby protecting the components of the pipette from contamination and corrosion, and more importantly, ensuring no cross-contamination between samples. Therefore, the filter tip is also an important tool for removing volatile and corrosive samples. Of course, the price of the filter tip is relatively high compared to the average tip, and the domestic market share is very low. Moreover, domestically produced filter heads are still few, and its filtering effect is also unsatisfactory.

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