How to choose a centrifuge that suits the industry requirements?

First, select according to the required centrifugal force:

Centrifuges use the principle of centrifugal sedimentation to separate, concentrate or purify cells (particles) with different densities in solution. The centrifuge tube with the same amount of test solution is placed symmetrically in the rotor (test tube). After the instrument is started, the relative centrifugal force ( RCF ) generated by the motor rotating the rotor at high speed separates the cells (particles) with different densities in the test solution. The magnitude of the centrifugal force depends on the horizontal distance from the position of the sample to the axis, ie the radius of rotation r and the speed n .

Its calculation formula is as follows:

RCF = 1.118 × 10 ^-6 n ² r × g

n------------ Speed ​​(rev / min)

r------------ Rotation radius (mm)

Second, according to the separation workload and research needs to choose:

1. Generally use benchtop centrifuges for research and small batch preparation;

2. Large-scale floor-standing centrifuges are used for large-scale preparation;

3. The county-level dimethyl-level laboratory or more uses a refrigeration system, a desktop high-speed refrigerated centrifuge or a floor-standing low - speed refrigerated centrifuge , and the township medical unit selects a desktop high-speed refrigerated centrifuge;

4. Blood centrifuges should be used in medical units with large blood banks.

  Third, select according to the required temperature of the sample:

1. Separate samples can be stored at room temperature +15 without changing their characteristics. Normal temperature centrifuges can be used.

2. If the separated sample is affected by temperature and its characteristics (such as proteins, cells, etc. ) will be destroyed in a high temperature environment, it is necessary to select a refrigerated centrifuge, which has a rated temperature range.

3. Some samples need to be heated according to the test requirements (such as oil, milk fat), you need to use a heated centrifuge.

Fourth, according to the brand selection:

(1) Pay attention to the origin: The main production centers of domestic centrifuges are Beijing, Changsha, Hunan, Anhui, Shanghai, etc., especially in Changsha, Hunan.
(2) Pay attention to the brand: There will be many manufacturers of different brands in the main production and distribution centers. Many manufacturers are keen to hang up the evidence to demonstrate the virtual strength: to continue the glory of the state-owned old factory, double high-tech personnel and so on.

(3) Pay attention to the styling: Many manufacturers are actually small assembly plants of the 3rd and 5th shareholders. They have neither R&D capability nor control technology, and their own strength is insufficient. The use of public molds to go through homogenized production routes, resulting in products on the market. It is the same as the shell shape.

Five, the choice of turning

Rotor: The rotor of the centrifuge is mainly divided into two types.

Horizontal rotor: The running blue is horizontal when running, at right angles to the rotating shaft, and the sample concentrates the sediment on the bottom of the centrifuge tube.

Angular rotor: The centrifuge vessel is at a fixed angle to the shaft, and the sample concentrates the sediment on the bottom of the centrifuge tube and the side wall near the bottom. If you want the separated sample to be concentrated at the bottom of the centrifuge tube, select the horizontal rotor. If you want the sample to concentrate on the bottom of the centrifuge tube and the side wall near the bottom, select the angle rotor.

There are also special tests or special samples that require special rotors such as large-capacity baskets, microplate rotors, slide rotors, PCR rotors, test tube rotors and capillary rotors. The rotor has a fixed size, which is combined with the capacity of the centrifuge, such as a 36 × 5 ml angle rotor, which determines the type of rotor and determines the capacity of the centrifuge, so the choice of the rotor is very important.

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Hunan Huxi Instrument Equipment Co., Ltd.

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