CultrexCoat® Cell Invasion BME Concentration Optimized Tumor Cell Behavior Analysis Protocol

Cell migration is the movement of a cell after it receives a migration signal or senses a concentration gradient of certain substances. During the process of cell migration, the cells repeatedly repeat the process of extending the protruding body to the front and then pulling the cell body. In tumor cell behavioral analysis, cell invasion is a prelude to tumor cell metastasis, and metastasis is the continuation and outcome of tumor cell invasion. Cell invasion refers to the phenomenon that malignant tumor cells are abnormally distributed in the interstitial space in terms of quality and quantity, and is a manifestation of a series of processes such as tumor cell adhesion, enzymatic degradation, movement, and proliferation in the matrix.

At present, the traditional cell invasion analysis method is to perform cell behavior analysis through the upper and lower chambers of the invasion chamber containing the carbonate filter. However, these traditional methods of cell behavior analysis have been criticized by researchers because of the following defects: 1. Higher experience on the operator's experience, such as uniform deposition of matrix proteins and late cell counting, it is difficult to achieve higher The stability of the experimental batch; 2, the cell matrix used for purchase did not undergo strict quality verification, which had a great influence on cell behavior and even activity, and the final data lacked credibility; 3. There was a certain experimental randomness in manual cell counting. . Here, Agilent Technologies recommends Trevigen's CultreCoat® and Cultre® full range of tumor cell behavioral assays to provide cell invasion, cell migration, cell adhesion, in vitro vascular regeneration, and vascular permeability analysis. And other complete tumor cell behavior analysis products and kits.

For cell invasion assays, Trevigen also offers two assay kits: the CultreCoat® range of pre-embedded BME matrices and the Cultrex® range of different matrix proteins. Among them, the CultreCoat® series provides pre-embedded matrix plates of different concentrations of BME, and the operator can directly select the appropriate concentration of BME matrix for direct use in the experimental points. Since different cell lines and different treatment methods can produce multiple invasion possibilities, each matrix is ​​optimized by adjusting the substrate concentration to make it suitable for each experiment. Therefore, we recommend CultreCoat® BME pre-embedded cell invasion analysis optimization. The sample selection kit obtains the most appropriate BME concentration protocol for different cells to obtain the best invasive experiment results.

The cell invasion analysis solution provided by Trevigen has the following characteristics:

1. Researchers can purchase CultreCoat pre-embedded kits according to their own application and needs, or choose the matrix components of different components of Cultrex (operators can adjust the substrate concentration according to different applications to optimize conditions), or Purchase only Cultrex basement membrane extract components that have undergone rigorous biofunctional testing;
2. Because different cell lines and different treatment methods can produce multiple invasion possibilities, it is necessary to select the most suitable concentration of BME as the basement membrane for cell invasion analysis for different cell sample types. Trevigen also provides pre-embedded cell invasion BME concentration optimization. The sample selection kit is designed to select the right BME concentration;
3. Trevigen's Cell Invasion Protocol provides fully quantified cell infiltration analysis without manual cell counting; pre-embedded Trevigen's patented BME matrix and other component proteins on microplates with extremely low cytotoxicity The absence of bacteria/fungi/mycoplasma, etc., is close to the real in vivo environment; at the same time, the fluorescence quantification scheme guarantees higher precision.

The CultreCoat® Cell Invasion BME Concentration Optimizer Kit is designed to select the right BME concentration. The microplates provided in the kit also contain pre-embedded lower, moderate and higher concentrations of BME, according to the protocol. After the invasion experiment, the optimal BME concentration regimen can be obtained according to the difference of invasion at different concentrations. The kit includes pre-embedded BME (or different matrix proteins) cell invasion/migration assay plates, matrix protein coating buffer, cell eluate, cell separation solution, and fluorescent dye Calcein-AM. Both 24-well and 96-well formats are available, and each well is pre-embedded with low, medium and high concentrations of BME for optimal screening of BME concentrations.

Agilent offers you a one-stop cell invasive analysis product and protocol, including the CultreCoat® series pre-embedded with different concentrations of BME matrix and the Cultrex® series of cell invasion assay kits with separate matrix protein components; if you wish To obtain the most appropriate concentration of BME for invasive analysis, you can choose the Cell Invasive BME Concentration Optimization Kit to select the optimal concentration of BME for your cells to be tested. In addition, Amy Jet also offers Cultrex Base Membrane Extract (BME) and individual component protein products from Trevigen and Southern Biotech. If you are interested in the above products and solutions, please contact Ai Meijie Technology Co., Ltd. for laboratory solutions related to cell adhesion analysis, or request the latest product information.

Trevigen is a fast-growing US biotechnology company focused on oncology research products and services for cell-wide analysis, apoptosis, DNA damage and repair, tumor cell function and behavior. As the general agent of Trevigen in China, Amy Jie and Trevigen provide Chinese researchers with the best and latest quality products and technical services in the fields of oxidative stress, cell damage and tumor cell behavior research.

Medical Equipment

Medical Equipment,Medical Dental Unit,Dental Medical Equipment,Medical Dental Equipment

ZHEJIANG FOMOS MEDICAL TECHNOLOGY CO.,LTD. , https://www.ifomos.com