This situation is mainly caused by improper management of the mushrooming period. The following are reasonable management measures: 1. Bud period. Adopt budding management measures, open the doors and windows of the mushroom house, enhance the light and ventilation, and spray water into the space to increase the relative humidity of the air in the mushroom house to about 90%, cool down to about 10 ° C, and loosen the bag mouth to induce mushroom bud However, at this time, the plastic film at the mouth of the bag must not be fully stretched to prevent evaporation of the culture material and affect the formation of mushroom buds. A few days later, when cotton-like hyphae or yellow water droplets appeared on the culture surface, the mouth of the bag was opened, and after a week or so, needle-shaped mushroom buds gradually grew. 2. Domestication period. When the fruit body grows to about 1 cm, the water spray should be reduced or stopped, and the humidity should be controlled at about 75%. Ventilate and cool down, adjust the temperature to about 5 ℃. After domestication, restore and maintain the temperature of the mushroom house at about 10 ° C to promote fruit body growth. 3. Elongation period. Measures should be taken to promote the rapid extension of the stalk. (1) Sleeve and straighten the mouth of the bag. When the fruit body grows to 2-3 cm, the excess film at the mouth of the plastic bag should be stretched and straightened. This can limit the oxygen supply and increase the carbon dioxide concentration. The purpose is to inhibit the umbrella cover from opening and promote the stalk extension. (2) Temperature adjustment. The temperature is controlled at about 10 ° C. (3) Humidification. The relative humidity of the air should be maintained at 85-90%. The mushroom room should be sprayed with water to keep it moist. The amount of sprayed water will increase as the fruiting bodies grow. (4) Low light induction. Above the middle of the two rows of bed frames, a 15-watt light bulb is installed every 3 to 5 meters to generate vertical light, which induces the fruiting bodies to reach the direction of the light source in a bundle and promotes the elongation of the fungus stem. (5) Regulation of carbon dioxide concentration. By reducing the number and time of ventilation, the carbon dioxide concentration in the air of the mushroom house is maintained at 0.1% to 0.15%. Through the above management measures, after about 15 days, a flammulina velutipes product with good commerciality can be produced with a diameter of about 1 cm and a stalk length of 8-15 cm. Disclaimer: Some articles on this website are transferred from the Internet. If you have third party legal rights, please inform this website to deal with them. phone Extraction Kit Magnetics Bead Method
This standard specifies the terms and definitions, classification, technical requirements, test methods, identification, labels and instructions for use, packaging, transportation and storage of nucleic acid extraction kits (magnetic beads method) (hereinafter referred to as "kits"). This standard It is suitable for extracting and purifying human genomic nucleic acid and its fragments from various clinical samples such as serum, plasma, whole blood, cerebrospinal fluid, milk, saliva, urine, sputum, swab, tissue or paraffin-embedded tissue by magnetic bead method , Pathogen nucleic acid kit. Pathogen nucleic acid includes deoxyribonucleic acid (DNA), ribonucleic acid (RNA), etc. This standard does not apply to nucleic acid extraction reagents contained in closed systems that cannot extract nucleic acid extraction products for detection.
This kit uses magnetic beads with unique separation function and a unique buffer system to separate and purify high-quality virus from serum, plasma, lymph, cell-free body fluid, cell culture supernatant, urine or various virus preservation solutions DNA/RNA. The uniquely embedded magnetic beads have a strong affinity for nucleic acids under certain conditions, and when the conditions change, the magnetic beads release the adsorbed nucleic acids, which can achieve the purpose of rapid separation and purification of nucleic acids. The whole process is safe and convenient, and the extracted viral DNA/RNA has high yield, high purity, stable and reliable quality, and is especially suitable for automated extraction of high-throughput workstations. The nucleic acid purified by this kit can be applied to various routine operations, including RT-PCR, fluorescence quantitative PCR and other downstream experiments.
Nucleic Acid Lsolation Reagent Kits,Dna Purification Kit,Viral Rna Extraction Kits,Nucleic Acid Extraction Reagents Jilin Sinoscience Technology Co. LTD , https://www.contoryinstruments.com