Treatment of Blood Samples of Experimental Animals-----Series IV

How to avoid hemolysis of blood specimens? What should I pay attention to?

Treatment of Blood Samples of Experimental Animals Series IV - Factors Affecting the Quality of Blood Specimens

1. The inner wall of the blood collection needle is not smooth

Blood collection needle classification:

1) Stainless steel needle: The outer wall is smooth, the inner wall looks smooth, the body is rough, and it is easy to scratch the cells, causing a hemolysis cascade reaction, resulting in hemolysis of blood specimens.

2) Venous retention needle: The outer wall is smooth and the inner wall is smooth, which can avoid scratching cells and effectively prevent hemolysis caused by blood collection process, but the cost is high.

2, blood specimens container is not clean

3, mixed into animal hair

The operation of the blood collection process is not enough and is not clean enough, causing foreign matter such as animal hair to enter or contact with blood specimens, causing hemolysis.

4, the pumping speed is too fast, the pressure is too large

The greater the withdrawal speed in the effective needle cross-section, the greater the pressure, the cells are squeezed, easily causing rupture, causing hemolysis.

5. The needle is not removed when the blood is injected into the container from the syringe.

When the blood is injected into the vacuum blood collection tube, the vacuum pressure is too large, causing the cells to burst and rupture, causing hemolysis. The needle should be removed and the blood injected slowly along the wall of the tube without foaming.

6, the formation of air plugs when collecting blood

The blood can only be pumped outwards and cannot be pushed into the blood vessels to avoid injecting air to form a gas plug, which leads to hemolysis.

7, the experimental factors

Hemolysis due to the experimental factors, such as chloral hydrate, has a slight hemolysis effect.

8, violent shock during transportation

Avoid hemolysis reactions caused by violent shock during the transport of blood specimens.

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