Recently, in order to quickly test the Ebola virus in the blood to minimize the risk of viral transmission, STMicroelectronics (ST), Clonit and the National Institute for Infectious Diseases (National Institute for Lazaros Palazzani) Infectious Diseases Lazzaro Spallanzani) After several weeks of collaboration, a prototype of a portable analyzer capable of detecting Ebola in 75 minutes was developed. The portable analyzer is based on a molecular biology technique of real-time polymerase chain reaction (RT-PCR). The next development of this collaboration is to optimize the point-of-care Ebola detection protocol, including minimizing the risk of infection during the processing of biological samples, while reducing costs. It will enable it to be widely applied on a large scale to speed up the process of rapidly diagnosing Ebola and other highly transmitted viruses. And the National Institute of Infectious Diseases in Palazzani, Lazaros, Italy, is one of two Ebola care and treatment centers designated by the Italian Ministry of Health; the prototype of the analytical tool suite has passed the international standard test developed by the center and can accurately The Ebola virus in the blood is found, and only a few milliliters of human blood sample is needed during the entire test, and the accuracy of the test results can be verified by diluting a blood sample of 1 million times. Because of its high sensitivity and accuracy, this solution can be used for early detection of viruses in the early stages of the disease, helping to significantly inhibit the spread and spread of deadly infectious diseases. The tool kit has 4 main components: · Extractor: store blood samples and extract RNA viruses; · Silicon microchip: A microlithic chip developed by Agfa's Agrate Brianza and Catania labs, which is only a stamp size, can act as a microreactor to reproduce the micron-scale amplification screening process for extracting genetic material. The extracted RNA is placed in a reactor and can be reversed into DNA and amplified according to RT-PCR detection methods. ·Specific reagents: Reactive reagents developed by Clonit, pre-positioned on microchips, performing quantitative real-time PCR (virus loading) processes in accordance with standards and control methods specified by international quality control regulations; ·Portable optical reader: A solution developed by STMicroelectronics to detect the presence of viral DNA in blood samples and send the data to a connected computer host. The computer host software processes the test data and displays it in graphical form. The processing result is displayed. In addition to accurate detection, the fast detection speed and small size make this analysis kit ideal for first-aid and off-site diagnostics outside the hospital. Giuseppe Ippolito, research director of the Spalanzani Institute of Infectious Diseases, stressed: "This test has a very important impact on health care, public health and public health. In fact, if patients infected with Ebola can be diagnosed quickly The hospital can immediately take quarantine measures to reduce the risk of the virus spreading among the population and immediately take appropriate treatment measures." Valerio Fabio Alberti, Special Commissioner of the Sparanzani Institute of Infectious Diseases, further added: "The successful development of this technology means that the Institute once again meets the requirements of the scientific community and national governments to achieve major breakthroughs in infectious disease prevention and control technologies. †Andrea Cuomo, vice president of advanced projects at STMicroelectronics, said: "We are honoured to be able to invest our innovation and strong technical resources in serving humanity and contributing to the fight against major infectious diseases that threaten all mankind. The French semiconductor research team once again proved its value and creativity, creating new opportunities in the field of microelectronics." Clorot CEO Carlo Roccio said: "I think this biomedical achievement is an example of successful collaboration between Italian state-owned and private research institutions." Now, working with other leading companies in the field, partners are evaluating an integrated, fully independent, fully automated disease detection system that enables multiple analyses of blood samples simultaneously. Combining portability, automation, integration, and time optimization can further reduce costs and achieve greater efficiency, opening up unlimited possibilities for future low-cost Ebola virus screening applications. Valerio Fabio Alberti of the Spalanzani Institute of Infectious Diseases said: "This major collaborative research and development results in inspiring our institute to pursue our mission more forcefully, develop and validate new technologies, and introduce these new technologies into the national health system. Assist in testing." Technical Description Polymerase Chain Reaction (PCR) can amplify the target biological material contained in a small amount of blood for detection, such as Ebola virus. After repeated heating and cooling, the system analyzes biological material based on biological markers placed on the microchip. Accurate amplification control is a key step in PCR detection methods. The low thermal temperature of the chip and the micro-capacity of the blood sample being tested reduce the reaction time; the temperature change cycle is accelerated, enabling complex biological substances to be rapidly amplified without affecting their accuracy and reliability.
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