The High Pure FFPET DNA Isolation Kit is based on the principle of extraction by spin column method, eliminating the need for DNA precipitation and organic reagent extraction. It is an ideal tool for rapid nucleic acid extraction. ï·The entire extraction process takes only 3 hours (including the dewaxing process); Figure 1: Obtaining high yield DNA from FFPET samples The bar graph shows the average DNA yield obtained from 5 um FFPET tissue section samples from 3 different breast or colon cancer xenograft tissues. In general, there is a certain variation in DNA yield between individual samples of different tissue sections. RESULTS: Four clear target bands appeared in all lanes, indicating that High Pure FFPET DNA Isolation Kit can extract high quality DNA from FFPET samples, which is suitable for CGH chip analysis, and second generation sequencing requires high quality of nucleic acid template. Downstream application. Figure 3: Extracting high quality DNA from samples to improve qPCR detection sensitivity As a nucleic acid template in a qPCR experiment, high quality DNA, that is, good nucleic acid yield, purity, and fragment size distribution, is required, and the target gene can be detected with high sensitivity and without bias. 5 ul of DNA eluate was taken and amplified by LightCycler 480 Real-Time PCR Instrument to detect CycA (442 bp) or myo (89 bp) gene fragments. Figure 4: Application of DNA to leading-edge applications such as methylation-sensitive high-resolution melting curve analysis (MS-HRM) DNA extracts from different tumor research samples were treated with bisulfite, and MS-HRM analysis of APC gene was performed by LightCycler 480 Instrument. The APC genes of different tumor tissues have different degrees of methylation. Results: DNA obtained by extraction with the High Pure FFPET DNA Isolation Kit is an ideal template for MS-HRM analysis. In this study, a total of 3 different degrees of methylation were found: APC methylation level of sample A was 10-100%, sample B methylation level was 0-1%, and sample C APC was not present. Methylation. product information product Catalog number Packing specification High Pure FFPET DNA Isolation Kit 06 650 767 001 50 extraction reactions. Read the original text: http:// Omega-3 Refined Fish Oil,Omega Refined Fish Oil,Refined Fish Oil 30/20Tg,Refined Fish Oil ZHOUSHAN SINOMEGA BIOTECH ENGINEERING CO.,LTD , https://www.sinomegabiotech.com
ï· Obtain high DNA yield and quality;
获得 Get powerful signals in downstream applications such as qPCR, chip analysis and sequencing. Figure 2: Extraction of high quality DNA from samples for a variety of downstream applications.
DNA was extracted from HT29 xenograft FFPET samples and subjected to multiplex PCR of 100, 200, 300, 400 bp fragment length amplicons.
Lanes 1 and 2: Dewaxing lanes using xylene 3 and 4: Dewaxing lanes 5 and 6 using hexadecane: multiplex PCR control, nucleic acid sample for complete genomic DNA extracted from human whole blood
Figure 2: High RNA yields can be extracted from both recent and long-term FFPET samples
RESULTS: DNA samples obtained from the High Pure FFPET DNA Isolation Kit were able to achieve higher detection sensitivity (Cq values ​​in advance) in qPCR studies.