ProcartaPlexTM performs analysis of more biomarkers in smaller samples. Developed by a superior immunoreagent supplier for the Luminex® platform, ProcartaPlex allows you to sample 25-50 μL (plasma, serum, cell culture supernatants and other body fluids). Simultaneous detection and quantification of up to 50 factors in 25-50 μL samples. This technique uses different stained capture microspheres for each target in a multi-channel "class ELISA" assay. Compared to traditional sandwich ELISAs, ProcartaPlex immunoassays can analyze up to 50 times the number of factors with a small sample size and the same time. ProcartaPlex Multichannel Immunoassay offers a variety of kit types covering six species (human, mouse, rat, non-human primate, pig, and canine) to meet your research needs. Proper sample collection and collation is a critical step in the success of the experiment. Currently ProcartaPlex can detect sample types: cell supernatant, serum, plasma. Although other biological samples have not been validated, they can theoretically be tested. Includes: BAL, saliva, CSF, tissue homogenate and urine. Below we will collect and save several common samples: When detecting secreted components, it is best to collect them in sterile tubes. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If you can, please follow the recommended method in the reference. After the specimen is taken, the liquid nitrogen is quickly frozen and stored for use. The specimen still maintains a temperature of 2-8 ° C after melting. Add a certain amount of buffer containing protease inhibitor and homogenize the sample by hand or homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully. If there is a sediment during storage, the sample should be centrifuged again to remove the precipitate before the test. It is recommended to centrifuge at 16,000 x g for 5 min. The above information is partly sourced from eBioscience. Read the original text: http:// Organic Chinese red dates are also called red jujube. It can be used for medicinal purposes. It has the effects of nourishing the stomach, strengthening the spleen, nourishing blood, nourishing, and strengthening the body. The date and root can be used as medicine. The date can be soothing and is one of the important drugs.
Organic jujube is warm and sweet, with the functions of nourishing blood, strengthening the spleen and stomach, and preventing wind. It is used to treat allergic purpura, anemia, hypertension, acute and chronic hepatitis, and cirrhosis in patients with elevated serum transaminases, and is an ideal choice for preventing blood transfusion reactions. Red Dates,Organic Chinese Red Dates,Dried Red Dates,Organic Jujube Dried Organicway (xi'an) Food Ingredients Inc. , https://www.organicwayinc.com 1, serum Whole blood is solidified at room temperature (20-25 °C) for 20-30min;
Room temperature 1,000 xg 10min;
Collect the supernatant or choose to use a serum separator;
Optional: If the sample is a hyperlipidemia sample, centrifuge at 2-8°10,000 xg for 10 min to separate the lower serum;
Multi-factor detection of serum samples requires freshness; if it cannot be detected within 24 hours, it needs to be dispensed below -20 °C to avoid repeated freezing and thawing. 2, plasma Citric acid or EDTA is selected as an anticoagulant as required. If heparin is selected as an anticoagulant, it is recommended that no more than 10 IU of heparin per ml of blood (heparin is too high, which may result in some high detection values).
After collecting anticoagulated whole blood for 30 min, centrifuge at 10,000 xg for 10 min at 4 °C, and collect and collect plasma;
Optional: Optional: If the sample is a high-lipid sample, centrifuge at 2-8°10,000 xg for 10 min to separate the lower plasma;
Multi-factor detection of plasma samples requires freshness; if it cannot be detected within 24 hours, it needs to be dispensed below -20 °C to avoid repeated freezing and thawing. 3. Cell culture supernatant 4, tissue samples