1. Turn on the power, confirm the voltage is 220V, high-purity nitrogen cylinder high pressure > 10 kg, low pressure is 4.55 kg, pay special attention to avoid false low pressure. 2. System, 9# bottle, 10# bottle, activation chamber, reaction chamber, and empty bottle for leak detection. The lower valve pressure is 11 psi and the upper valve pressure is 3 psi. The pressure drop after closing the valve should be <0.30 psi / 3 min. The empty bottle should be removed to remove the small disc in the center of the aluminum cover. 3. Install the synthetic reagent bottle, pay attention to whether the reagent in the bottle is enough for the synthetic amount, and whether the sealing ring is suitable. Connect the computer to the host. 4. Measure the flow rate of each synthetic reagent bottle. Adjust the lower valve so that the flow rate of each bottle meets the specified value. Finally, the time correction value T is calculated. 5. Edit the polypeptide sequence on the computer from N to C. Select the desired synthesis method from ABI Chemistry. After the computer is stored, the data is sent to the host and the T value is corrected on the host. 6. After the host self-test, the black zebra stripes are scaled and the calibration value should be 0-4096. 7. Install the amino acid vial, remove the small round aluminum in the center of the aluminum cap, and mark the inside to check if the discharge sequence matches the edited peptide sequence. 8. Review the previous sequence before starting the peptide synthesis, and if there are any omissions. And check: whether the waste bottle is too full, whether the high pressure and low pressure of the cylinder are suitable, whether the pressure of the upper valve and the lower valve are correct, whether the filter needs to be replaced, and whether the valve switch is in the press use state. 9. Press the begin switch on the host menu to start peptide synthesis. The room temperature at the time of synthesis was 18-25 °C. 10. After the synthesis is complete, flush the system with 9# and 10# bottles of solvent and cylinder nitrogen. Remove all synthetic reagent bottles and seal with a clean, empty bottle. The valve switch is in the Vent position and the main unit is turned off. Fire Hose Reel And Cabinet,Fire Hose Reel ,Hose Reel Drum,Hose Reel Cover NINGBO TOMAN IMP. & EXP. CO., LTD , https://www.tdotmfiresolution.com