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First, materials and methods
1. Test sites and materials tests were conducted at Rudong Xinxing Aquatic Products Co., Ltd. Nursery pond (also known as the nursery pond for nursery) 700 square meters (35 square meters/piece, 20 pieces), single cell algae cultivation pool 320 square meters (20 square meters/piece, 16 pieces), the inner wall and bottom of the pool are made in Taiwan. Non-toxic paint smear, equipped with water, electricity, gas and other facilities.
2. Pro-Bell Origination and Fertility Production At noon of August 15, 2003, the shells in the Dongling Scallops and Mixed-breeding ponds in Rudong County were about 3.5 centimeters in length and about 140 capsules in kilograms per kilogram. When the mottled sex is mature, the female gonad is milky white and the male gonad is pale yellow. Pro-shell cleaning, sanding, washing with potassium permanganate disinfection, dry to the morning of the 16th, nursery ponds filled with sand filter seawater, pro-shellfish placed on the spawning grid, placed 25 cm below the surface of the inflatable stimulation Efforts were made to stop production and observations were stopped once every two and a half hours. After the spawning began, the inflating was stopped, the inflating hatching was completed after the oxytocin production, and EDTA disodium 5 g/m 3 was added to the seawater. Until 21:30 in the evening, a total of 4 billion eggs will be fertilized. The urine production temperature is 25.6 to 25.8°C.
3. Incubation and selection of seawater after dark precipitation over 24 hours, after the second sand filter with 1000 mesh filter bag filter. Fertilized eggs were hatched in the original pool and antibiotics were added at 1 g/m3. Take natural water temperature (25～26°C) to hatch, incubation time is more than 15 hours. After the D-shaped larvae of each pool were changed on August 17th, they were selected for transfer pool selection. The selected larvae with good vigor and strong floating ability were used for culturing.
4. The larvae were cultivated using a conventional method of bivalve cultivation. Seawater salinity 25-30, water temperature 25-26 °C, pH 7.8-8.3. The D-shaped larvae were fed with golden algae as an open bait. Three days later, Chaetoceros capillaris was added. After the larvae reached 160 micrometers or more, the algae, Chaetoceros and Platymonas were mixed and fed. The feeding amount of the unicellular algae is controlled according to the larva stomach fullness, and the amount of monocular algae is maintained at 20,000 to 50,000 per milliliter. Change the water 1 or 2 times a day, change the water daily by more than 50%, and remove the pool every 2 to 3 days. Daily feeding 2 to 3 times.
5. Single-cell algae cultivation Efforts should be made to cultivate single-cell algae before breeding, and to select algae species for preservation. The algae culture adopts the three-stage culture method. The seawater of the first and second cultures is disinfected by high temperature, the seawater of the third-grade culture is treated with bleaching powder of 100 g/m3, precipitated for more than 24 hours, neutralized with sodium thiosulfate, and the light is strictly controlled. , aeration, nutrient salts, etc., to ensure the normal production and supply of monocytic algae culture, ensuring smooth progress of nursery work.
6. Metamorphic attachment and juvenile rearing The method of attaching and culturing the sandy bottom material is used. Take clean and uncontaminated sand mud by seawater disinfection after boiling, and use 100-mesh sieve to filter after use, each pool of 2 to 3 tablespoons, evenly thrown at the nursery bottom. The sand belt seedlings are washed once every 3 days. The amount of water changed every day reaches 100%, and the water level is controlled at a depth of 60-80 cm.
7. Daily management Daily water temperature, specific gravity, pH and other water quality factors were measured. At the same time, the larvae and juveniles were observed for their fullness and growth, and their routine work was recorded.
Second, the results
1. Incubation and selection of the original pool hatch, the water temperature is 25.6 ~ 25.8 °C, hatching density of 30 / ml. From 17 o'clock on August 16 to 12 o'clock on August 17, the D-shaped larvae in the four pools gradually became homogeneous. After selection, 3.5 billion D-shaped larvae were obtained, and the hatching rate was 87.5%.
2. After larvae were cultured, D-shaped larvae were selected and transplanted into 7 nursery ponds for cultivation at a density of 10-15/ml. The larvae grow an average of about 10 microns per day. After about 10 days of incubation, most larvae go into metamorphosis. The growth of D-shaped larvae is shown in Table 1.
3. Metamorphosis and juvenile culture The larvae that have just entered the metamorphosis attachment stage have an average shell length of 185 microns and a number of about 3 billion grains. The juveniles are cultivated using a sand-bottomed method, which is usually washed once a week or so. The initial control of juvenile rearing density was less than 2 million square meters, and gradually reduced the cultivation density. After more than 20 days of cultivation, the number of juveniles with a shell length of 300 microns or more was 2 billion tablets on September 9. The survival rate of larvae of D. variegata larvae to 300 m in shell length was 57%.