In the production process of edible fungi, seed production is a key link. In the production process of strains, improper operation and incomplete disinfection will lead to the invasion of miscellaneous bacteria, contaminating the strains, reducing the edible fungus commodity rate, and the benefits. The measures are described as follows: 1. Penicillium is the most common type of miscellaneous bacteria. After contamination, it appeared as a small, individual green powder on the medium. Mildew. The bacteria are widely distributed and spread by conidia in the air attached to moist materials without thorough disinfection. Prevention and treatment measures: Strict disinfection of culture materials; strict control of aseptic operation techniques during inoculation; strengthen ventilation, cooling, and humidity reduction; local pollution can be washed with 5-10% lime water. 2. Trichoderma is mainly Trichoderma viride or Trichoderma koningii. It relies on spores that are produced in rotten wood, seeds, plant residues, compost and soil, and organic spores that float in the air for transmission. The fungus is acidic. It can easily occur when the temperature reaches 25-30 ° C and the relative humidity of the air is 80-90%. It can develop from spotting to the whole surface in 3 to 5 days. Control measures: (1) Strict disinfection of the culture material to completely eliminate the mycelia and spores in the culture material; do aseptic operations, strengthen ventilation and cool down. (2) Do not use too much formaldehyde during disinfection to prevent acidic environment. (3) When there is Trichoderma on the culture medium, apply 755 alcohol, 15% bleach powder solution, and 50% carbendazim 200 times solution in time. Spray 1 to 5% of lime water or dust the white powder, and then dig it out. Apply lime water. It is best to move the bacteria bag (bottle) outdoors when processing to prevent the spores from flying. After the appearance of Trichoderma, it is strictly forbidden to open the bottle cap or peel off the film to avoid spreading. When using plastic bags for seed production, it should not be excessively moved during cultivation. (4) Remove ungerminated strains in time. (5) The tampons in the strain bottles and strain bags should not be damp, and the culture room should be kept dry and clean. (6) The mother seed must be pure. (7) When mixing, add 2 grams of 25% (or 5%) carbendazim or 1 grams of 75% nail polish per kg of dry material. 3. Mucor, Rhizopus and Aspergillus Mucor, also known as Trichophyton, mainly occur on the initial culture medium and species of indoor mushrooms (ears). The mycelium was white or off-white in the beginning, and then turned pale yellow. The mycelia are sparse and stout, and they can be seen clearly. The larger the moisture content of the culture material, the heavier the occurrence. Strong ability to break down starch and grow very fast. Control measures: (1) Thorough disinfection and sterilization, and do aseptic operation. (2) Add 2 grams of carbendazim 25% (or 5%) or 1 gram of 75% methyl tobbutin per kg of dry material when mixing. Fourth, Streptomyces is also called Neurospora, which can be harmful during the production of various strains. Especially when the temperature is high and the humidity is high. The fungus is widely distributed in nature, and all kinds of organic matter, such as corn cob, corn stalk, cottonseed husk, waste cotton, etc., are easy to occur. Its conidia are floating everywhere. Control measures: (1) Do a good job of environmental sanitation, and bury the waste culture material deeply. (2) The culture material is thoroughly sterilized, and the tampons should not be damp, and they should be replaced in time after being damp. Bacteria bottles (bags) should not be damaged. (3) The inoculation room should be thoroughly sterilized and strictly adhere to the aseptic procedures. (4) Sprinkle lime on the ground and lime on the tampon at the bottle mouth if necessary to keep it dry. After it is found, it will be rejected, burned, and sprayed with 75% wettable tobbutin 500 times liquid, or fumigated with 10 ml formaldehyde per cubic meter of space. Disclaimer: Some articles on this website are transferred from the Internet. If you have third party legal rights, please inform this website to deal with them. phone
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