Male-derived rice embryogenic triggers are vegetatively propagated by seeds

December 26, 2018 Source: Ministry of Science and Technology

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On December 12, researchers from the University of California and the University of Paris-Sacré in France published an article entitled "A male-expressed rice embryogenic trigger redirected for asexual propagation through seeds" on Nature, which found rice (Oryza sativa). BABY BOOM1 (BBM1), a member of the AP2 transcription factor family expressed in sperm cells, plays an important role in the initiation of embryogenesis.

The molecular mechanism by which flowering plants trigger embryogenesis after fertilization and how to avoid re-fertilization is unclear. In the present study, the researchers found that parthenogenesis can be performed by ectopic expression of BBM1 in rice egg cells, suggesting that a single wild-type gene can “bypass” the fertilization checkpoint of female gametes. The expression of BBM1 in the fertilized egg is first derived from the male allele, followed by the parents. The triple knockout of the BBM1, BBM2 and BBM3 genes resulted in embryonic development arrest and seed abortion, whereas BBM1 from male expression completely rescued this phenomenon, suggesting that embryonic development is mediated by male genomic pluripotency factors during fertilization. Using the CRISPR/Cas9 genome editing technique to knock out three genes associated with meiosis (MiMe) to obtain diploid gametes that produce mitosis similar to somatic genotypes, and ectopically express BBM1 in egg cells, A cloned progeny that maintains the parental heterozygosity of the whole genome can be obtained. This artificial asexual reproduction trait can be multi-generation asexual inheritance.

Due to genetic separation, the high-yield characteristics of hybrid crops are often not transmitted through the offspring. This study confirmed the feasibility of asexual reproduction of crops and the ability to maintain the asexual reproduction of hybrids by seeds.

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