Recently, fruit farmers everywhere have reported that early defoliation in orchards is seriously harmful and difficult to control. To this end, the reporter interviewed Zheng Ke, a lecturer at the Zhongnong Le Fruit Industry Research Institute. He introduced that in the past few days in Shanxi Linyi Beixin, Zhuoli, Wanrong Wangxian, Gaocun, Han Xue and Yongji, Salt Lake, Yucheng Some fruit area surveys found that the main cause of yellowing and early fall of this year is brown spot disease, and other parts of the orchard are also spotted deciduous diseases and rust. The factors that have caused these diseases to occur this year are as follows: More pathogens Last year, due to the high temperature and rain in the later period, many orchards appeared a lot of defoliation in September-October, and in October, the fruit harvesting period was approached. Many fruit farmers adopted a “tolerance†attitude and did not actively control it, thus causing the bacterial base in spring this year. Significantly increased. Rainy and humid Last winter, the Qingyuan Garden was not thorough. It was wet and rainy this spring, which created conditions for brown spot disease, spotted leaf disease and rust. There are more rains this spring, which is conducive to the expansion and infestation of the conidia of these germs, so the incidence is earlier and heavier than in previous years. Do not take the right medicine in time Some fruit farmers have poor awareness of prevention and treatment. They do not need medicines when they are sick. When they are sick, they have to use medicines. The loss has been caused, and the difficulty of prevention and treatment is increased. When the medicine is used, the cost of the medicine is high because of the low cost of the medicine, and the special medicine is not used. Prevention and treatment, with other agents instead of achieving the control effect. There are also some fruit farmers, no matter how the climatic conditions change, dead and hard, using some cheap package drugs, prevention and control is not in place to cause a lot of leaves. Rain after spraying This year, due to the rain, the rain showers in the 12 hours after the spraying of the fruit trees are not common. Most people generally do not re-spray, so that the bacteria can not be effectively killed, but it is easy to produce drug resistance. Heavier ringing Some fruit farmers are eager to seek success, and the ring stripping is heavier. In addition, the high temperature and high humidity wounds are not ideally healed, resulting in weak tree, coupled with canopy closure and poor ventilation, resulting in easy infection of orchards. For the prevention and control of brown spot disease, the protective fungicide and the therapeutic fungicide should be alternately sprayed, and the Bordeaux mixture, pyraclostrobin, etc. can be used, and the liquid must be sprayed and sprayed. At present, for spotted leaf deciduous disease, spraying a drug at the early stage of autumn shoot growth can control the disease again, and 70% mancozeb 600 times solution can be used. Disclaimer: Some articles on this website are transferred from the Internet. If legal rights of third parties are involved, please inform this website. phone
MLPA ( Multiplex Ligation-dependent Probe Amplification) method detects multiple copy number changes of genes or loci sites. Nowadays, MLPA is used to check large numbers of hereditary disorders and tumour profiling.Since Dutch Scientist Jan Schouten first invented it, the MLPA method was first published in 2002 'Nucleic Acid Research'. The principle of the MLPA is to apply the specific probe design targeting a region of interest on each sample DNA. MLPA method consists of the following steps:
Denaturation > Hybridization Ligation > PCR MLPA Amplificons Capillary Electrophoresis >Data Analysis
PCR reaction consists of the three steps: denaturation, primer annealing and primer elongation. Those steps of PCR amplification are repeated many times. The fluorescence-labeled primers, which will go through the capillary electrophoresis to pass a detector, are incorporated into the size of the amplification products. The measured fluorescence was visualized as a peak pattern, the so-called electropherogram. The raw data from the capillary electrophoresis analyzer forms the input of the MLPA analysis.
The genetic analyzers of Superyears Gene can be used not only for Sanger sequencing but also for fragment analysis. Fragment analysis First, it obtains the fluorescence-labelled DNA fragments, performs the capillary electrophoresis using a Genetic Analyzer and compares the sample's relative size standard with the designed size standard markers through the analysis software.
The genetic analyzer of Superyears Gene can be used in the capillary electrophoresis during the process of the MLPA experiment, and the data obtained can be used for (compatible) professional analysis software.
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