1. Explain the use and restrictions of the safety cabinet to all users who may use the biosafety cabinet in accordance with national standards and relevant regulations. A written statement or safety manual or operating manual should be issued to the staff. In particular, the staff must be clearly told that the safety cabinet cannot protect the operator in the event of spills, crushing, or improper methods. 2. The safety cabinet can only be used when it is working properly. 3. The glass observation window on the front of the safety cabinet must not be opened when the cabinet is in use. 4. The instruments and materials placed in the cabinet must be kept at a low quantity. The air circulation at the rear of the static pressure box must not be obstructed. The material should be decontaminated before it is placed in the work area of ​​the cabinet. 5. Bunsen burners (a type of gas lamp) must not be used in the cabinet, as the heat generated may interfere with the airflow and may damage the filter. Micro-electric incinerators can be used, but it is better to use disposable sterilization rings. 6. All work in the cabinet should be carried out in the middle or rear of the work surface and can be seen from the viewing panel. 7. The activity behind the operator should be less than Zui. 8. The operator should not extend the arm back and forth out of the cabinet to avoid disturbing the airflow. 9. The forward style grille must not be blocked by notes, droppers, or other materials, otherwise it will interfere with the airflow and may contaminate the material and expose the personnel. 10. Clean the surface of the cabinet with a suitable disinfectant after each work and at the end of the day. 11. The fan of the cabinet should be operated for 5 minutes before the start of work and after the work is completed. Biosafety cabinet placement Gas flow rate flowing into the cabinet from the front opening of the cabinet biological face about 0.45 m / sec. At this wind speed, the consistency of the one-way flow in the cabinet is easily disturbed, and it is easily disturbed by the walking, windowing, air distribution, and door switches of the personnel near the safety cabinet . In an ideal arrangement, it should be placed away from activities and where there may be interference with airflow. Try to leave 30 cm of space on the back and sides of the cabinet to facilitate maintenance. A height of 30 to 35 cm is left above the cabinet to accurately measure the wind speed of the exhaust filter and leave enough space for the replacement of the exhaust filter. Cleaning and disinfection of biological safety cabinets All items in the biosafety cabinet, including equipment, should be surface decontaminated and removed from the cabinet after work is completed, as residual culture media may provide microbial growth conditions. The inner surface of the cabinet should be decontaminated before and after each use. To wipe the surface of the table and the inner wall with a disinfectant, the disinfectant used should be able to kill all microorganisms that may be present in the cabinet. At the end of the day, the surface decontamination treatment should be carried out after the Zui, that is, the inside of the workbench, each side, the back and the glass should be completely wiped. 70% alcohol can be used, a disinfectant that is effective against the target organism. If a corrosive disinfectant is used, it should be wiped twice with sterile water. It is recommended that the work cabinet be in working condition when it is cleaned and disinfected. If it is not in operation, it should be run for 5 minutes to remove the gas before the cabinet is closed. Decontamination treatment of biological safety cabinet Decontamination of Class 1 and Class 2 biosafety cabinets is to place an appropriate amount of paraformaldehyde (80% of the final concentration of paraformaldehyde in the air in the cabinet) on a hot plate or frying pan (from outside the cabinet) control). In addition, a hot plate or frying pan with 10% more hydrogen carbonate than paraformaldehyde is placed in the cabinet (controlled outside the cabinet). This second container should be covered with a cover that can be removed from a distance (for example, on a line that can be pulled from outside the cabinet). This can make the early neutralization of paraformaldehyde reach a small size. If the relative temperature is below 70%, open the container with hot water. If there is no front bulkhead, use a raw plastic baffle and tape it to the front face to ensure that no gas leaks into the room. When the paraformaldehyde in the pan has completely evaporated or the switch has been turned on for 1 hour, the switch is turned off. Leave the safety cabinet for a whole night and do not touch it. The lid of the second tray is then removed and the switch is opened and the ammonia bicarbonate begins to evaporate. At this time, turn off the switch of the plate, let the safety cabinet start working, and let the ammonia hydrogencarbonate circulate for 1 hour. The front bulkhead (or plastic baffle) can then be removed and the safety cabinet ready for use. Shanghai Sujing Industrial Co., Ltd. Soy Sauce,Soy Sauce For Resturant,Raw Sauce Soy Sauce,Soy Sauce Seasonings Chinese Seasoning (Shandong) Trading Co.,Ltd , https://www.zt-trading.com
　　
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