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First, prepare
1. Before commissioning the machine, it is best to open the machine half a day in advance and place it in a room at room temperature above 22 degrees, especially in autumn and winter (25 degrees is best).

2. Fill the empty bottle engraved with “Supply Reservoir” with deionized water, and place the empty bottle engraved with “Waste Reservoir” as shown below, and tighten the lid. The desiccant is inserted into the tank, and the desiccant is not divided.




3. Turn on the power switch until “READY TO USE” is displayed.




Second, calibration
1. Press the “OPEN/CLOSE” button and the sample cell will be transferred to the position shown below.




2. Place a piece of filter paper with a pair of tweezers into the groove of the sample cell and make sure that only one piece is placed.

3. Pipette 10uL 290mmol/kg standard solution and add it to the filter paper.

note:
1. If using the original quantitative 10uL pipette of the manufacturer, it is not necessary to adjust the amount of sample to be used directly.
2. When adding standard solution or sample, please add it to the filter paper, do not drop on the groove edge of the sample cell. If it has been dripped on the groove edge, wipe it off immediately.

4. Press the “OPEN/CLOSE” button and the sample cell is closed.
5. Wait for the progress bar on the screen to go to the end point and display the measurement results.
6. Press the “CALIBRATE” button to calibrate the reading to 290mmol/kg.

7. Press the “OPEN/CLOSE” button, the sample cell is turned out, the filter paper is removed with tweezers and discarded, and the sample cell is wiped clean with a paperless paper towel.

8. Repeat the steps 2-7 with 1000 mmol/kg calibration solution and 100 mmol/kg calibration solution.

9. If the reading error of 100 mmol/kg calibration solution is >2, the reading error of 290mmol/kg calibration solution is >3, and the reading error of 1000mmol/kg calibration solution is >5, repeat 2-8. step

note:
1, calibration, one must units, i.e. 290mmol / kg, 1000mmol / kg, 100mmol / kg, three-point calibration sequence is completed, such is not within the error range, then a re-calibration or rounds until The measured values ​​of the 3 points are within the error range. If one point is not within the error range, it should be recalibrated. It is not possible to calibrate 290mmol/kg first, continuously calibrate several times, within the error range, and then continuously calibrate 1000mmol/kg to within the error range, and then continuously calibrate 100mmol/kg to within the error range. This is wrong.


 
 
Third, measurement
 
1. Press the “OPEN/CLOSE” button and the sample slot will be rotated out.
 
2. Place a piece of filter paper with a pair of tweezers into the groove of the sample cell and make sure that only one piece is placed.
 
3. Take a 10uL sample with a pipette and add it to the filter paper.
 
4. Press the “OPEN/CLOSE” button and the sample cell is closed.
 
5. Wait for the progress bar on the screen to go to the end point and display the measurement results.

note:
1. If the sample is thick and can not be sucked up with a pipette, you can use a syringe that is injected on the gas chromatograph to draw 10uL. If not, you can use a normal 1mL syringe.
2. Please use the “Auto Repeat” function of the machine carefully, because as the sample is exposed for a longer period of time, the water in the sample will evaporate and the osmotic pressure of the sample will increase. If you must measure multiple times, repeat the sample measurement.


For detailed information on the osmometer, please refer to: http://

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