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The change of insulin secretion pattern that accurately reflects the function of islet β-cells is complicated, and there is a change in secretion amount and time limit. This secretion mode makes it difficult to describe the function of cells with the total amount of insulin secretion. The amount of insulin secretion is not the same as that of β-cell function, and the change of islet β-cell function is a dynamic change of “quality and quantityâ€. Because the secretion is the same, there may be differences in the peak time of secretion. For example, those with normal glucose tolerance are lower than those with impaired glucose tolerance. The lower insulin secretion is not the same as the β-cell function; the insulin secretion is higher than normal. Beta cell function is hyperactive. If the beta cell function is evaluated by the total amount of insulin secretion, misjudgment is likely to occur.
The use of an isolated islet perfusion system can comprehensively evaluate islet function from both the secretory phase and the secreted amount. It is an ideal detection method and can be widely used in islet basic research. 
The automated biological fluid perfusion system developed by Biorep Technologies Inc. and the Diabetes Research Institute at the University of Miami greatly simplifies the experimental process and achieves comprehensive environmental control and stimulation. Different cell types and automatic collection of secretions in compliance with programmable protocols. This system significantly enhances the processing power and reproducibility of cell secretion analysis. The accuracy of the secretion experiment is greatly improved, and the systematic error of the experiment is reduced. 
At present, many famous universities (Harvard University, Yale University, Stanford University, Oxford University, etc.) and pharmaceutical companies (Pfizer, Novartis, Eli Lilly, Novartis, Takeda Pharmaceutical) have purchased Biorep's biological fluid perfusion system for research. . Furthermore, the Biorep biological fluid perfusion system is not limited to studies used for islet secretion. It can also be used for secretion studies of other types of cells, including cells such as stem cells, fat cells, chromaffin cells, and stem cells. This makes it an indispensable part of the pharmaceutical industry's drug development process. 
An important aspect of assessing islet function is to detect hormones and other substances secreted by islet cells under various stimulating conditions, which is especially important for the study of islet physiology.
The Biorep Biore Perfusion System is a product developed to meet this demand. It allows automated cell secretion analysis of secreted cells.
In addition, Biorep has partnered with Thermo Fisher Scientific to incorporate the Orbitor RS microplate transport arm as part of a new perfusion system. This creates a fully automated, high-throughput cell secretion collection system.
Fully automatic biorep biological fluid perfusion system
After rigorous experiments, the Biorep biological fluid perfusion system has proven to be very valuable. It can be used to detect the activity of human islets in an extracorporeal chamber before islet transplantation, and accurately reflect the timeliness of insulin secretion.
Biorep product technical features:
1 Up to eight different stimuli can be used in the same experiment
2 Automated multi-channel valves can select the desired source of stimulating fluid for each of the four repeating outflow channels according to a pre-set experimental method
3 High-precision peristaltic pump pumps the irritant from the source of the stimulating fluid to the perfusion chamber
4 During the study, the eight perfusion chambers acted as fixed cells. In addition to stimulating the cells, the perfusate washed away the metabolites secreted by the cells.
5 All stimulating fluids are in a closed container, just like a perfusion chamber, ensuring temperature uniformity.
6 The effluent was collected in a multiwell plate for further study. The collection plate is automatically transferred and replaced depending on the number of perfusion chambers used and the pre-set sampling rate.
7 The perforated plate is in a cool state, avoiding degradation of the analyte in the effluent
Product application status
The perfusion system was originally used to detect the activity of islets in vitro. Since then, its application has expanded to detect the secretion of differentiated cells derived from islet progenitor stem cells.
Recently, another application was discovered. The biorep perfusion system is coupled with tandem mass spectrometry to identify new secreted proteins during islet stimulation.
One of the primary advantages of perfusion culture and batch culture is when studying ion channel and receptor-mediated drug effects. In perfusion experiments, the effluent continually rinses out metabolites secreted by cells or cell populations during the study, and in batch culture, these metabolites accumulate in the incubation buffer.
This aggregation usually changes the characteristics of the signal transduction of the receptor and ion channels. For example, if the drug target being studied is an ATP-sensitive potassium channel, the zinc ions and insulin secreted by the islet beta cells are likely to alter the true effect of the drug on the channel. Because insulin and zinc ions are involved in the regulation of the affinity of ATP-activated ATP-sensitive potassium channels.
About Biorep Technologies Inc.
Founded in 1995, Biorep Technologies Inc. is an original medical device manufacturer. His product was originally developed to help doctors perform islet isolation and islet cell transplantation. Biorep's instruments are now widely used in diabetes research laboratories in the United States and around the world, and Biorep has been continually striving to design unique tools for accelerating diabetes research for therapeutic purposes.
For more information on the Biorep Biofluid Perfusion System , please visit or
Biorep in China - Shanghai Yi and Biology
Shanghai Yihe Biotechnology Co., Ltd. was established in 2010 and provides technical support from researchers from the Chinese Academy of Sciences, Peking University, Zhongshan University, Fudan University, Shanghai Jiaotong University, Tongji University, Zhejiang University, Xiamen University, Nantong University and other life science institutions. Theoretical guidance. Mainly engaged in molecular biology, cell biology, immunology, neurobiology, epigenetics, proteomics and other experimental research related reagents, consumables and equipment sales, is committed to providing customers with world-leading level and quality stability The product. Acting brands: American Biorep , Germany ibis diibidi, Germany Prand BRAND, American Biorep, American Frequency Devices, the United States Ames AMMEX.