Summary: Triacontanol has the functions of promoting rooting, germination, flowering, stem and leaf growth and early maturity, and has many physiological functions such as increasing chlorophyll content and enhancing photosynthesis. Appearance is white scaly crystal, melting point range 85.5-86.5 ° C, insoluble in water, difficult to dissolve in cold methanol, ethanol, acetone, soluble in ether, chloroform, carbon tetrachloride, no UV absorption. In the fully automated preparation and purification process, an evaporative scatter detector-liquid phase preparation system (ELSD-LC) is used for the non-UV absorbing substances (such as alkanols ) , and a good online process detection (PAT ) is obtained. ) The effect. 30-alkanol crude point plate analysis: Analytical method: TLC thin layer chromatography Point plate liquid: petroleum ether: ethyl acetate = 5:1 sulfuric acid color development Sample solvent: chloroform heating (40 degrees Celsius), It is obvious that you can see 3 points on the board, and the third point is trailing. experiment procedure: Weigh b. Heat in the water bath until the crude product is dissolved c. Weighing d. The chloroform in the spinner flask is evaporated to dryness to obtain a dry filler. e. Take 1/3 into the pre-packed column and install it on the prepared liquid phase instrument to prepare for separation. Preparation equipment: APPS 100D Column: Detector: Evaporative Light Scattering Detector (ELSD ) Elution flow rate: 10ml/min Mobile phase: petroleum ether, ethyl acetate Gradient elution: time min A phase B phase 0 100 0 1 100 0 1 95 5 41 75 25 The ELSD map was prepared as follows: A total of 4 peaks were separated from the test, and there were only 3 peaks on the spot plate. Below we confirm the four peaks again: After the board is clicked, the map of the board is obtained as follows: Three points on one board are displayed on the board, the peaks 1 and 2 are on the leading edge, the third peak corresponds to the second point of the crude product, and the fourth peak corresponds to the third point of the crude product. This is very clear. Achieved the purpose of the experiment! in conclusion: Through the above experiments, it can be seen that the alkanol can be detected by a liquid phase using an evaporative light scattering detector (ELSD) instrument without ultraviolet absorption. frozen IQF red shrimp Iqf Red Shrimp,Frozen Iqf Red Shrimp,Fresh Iqf Red Shrimp,Delicious Iqf Red Shrimp Zhoushan Junwei Aquatic Products Co., Ltd. , https://www.junweiaquatic-intl.com
