1. Ear room settings: professional cultivation room shed size to 9m4m4m (length, width and height) is appropriate, you can also use the housing as a cultivation room. In the room, 8-11 shelves are used, and 3000 ear bags can be discharged at a time. Each batch of production cycle is 35-40 days. 6 batches can be cultivated each year to form a multi-layered three-dimensional cultivation.

2. Cultivation season: Tremella belongs to medium-temperature fungi, the optimum temperature for mycelial growth is 22-25 °C, and the optimum temperature for fruit body development is 23-25 ​​°C. During the cultivation season, the natural temperature in spring and autumn is the most suitable. It can also be heated in the winter cultivation room and cultivated in shade in the summer.

3. Culture medium preparation: The raw materials for cultivation of white fungus are very extensive, with cottonseed husks being the most ideal. In addition, miscellaneous wood chips, corn cob, and bagasse are also commonly used raw materials. The commonly used medium formula is: 1 cotton husk 85%, wheat bran 13%, gypsum powder 1.5%, sucrose 0.5%; 2 cottonseed husk 80%, wheat bran 15%, corn flour 3%, gypsum powder 1%, sucrose 1 %. When other raw materials are used for cultivation, the amount of wheat bran should be appropriately increased and the ratio should not be lower than 20%. After mixing the materials, the water content is 60% and the pH is 5.2-5.8.

4. Bagging sterilization: The cultivation bag is made of low-pressure polyethylene film bag, the bag size is 12cm-55cm, and 600g of dry material per bag. The front of the bag hit 3-4 inoculation points, and the hole was sealed with a tape. After bagging, it was sterilized under normal pressure, and the temperature rose to 100°C for 16-20 hours. Then the bag was discharged and cooled.

5. Inoculation seal: Tremella mycelium is a mixture of feather-like incense hyphae and tremella-silver pure mycelium. Inoculation requires strict implementation of non-defective operations. When the inoculation was started, the tape was opened and the Tremella species was extracted with an inoculum needle, and the hole tape was smoothly sealed. Each bottle of bacteria can receive 40-50 bags. Before the inoculation, the Tremella species should be mixed and mixed repeatedly so that the two types of mycelium can be mixed evenly to increase the ear rate.

6. Germ culture: The germ chamber requires clean, clean and dark. The bacteria bag enters the house and adopts overlapping discharges. The indoor temperature is controlled at 25-27°C for the first 3 days. If the temperature exceeds 30°C, the window should be opened for ventilation and cooling. If the temperature is lower than 18°C, the doors and windows should be closed to warm. The relative humidity in the bacteria phase is controlled below 70% to prevent moisture.

7. Open mouth base: After 10 days of incubation, when the diameter of the mycelium circle is more than lOcm, the bacteria bag can be moved into the sub-cultivation room, and the opening is oxygenated, and the trachea is drained to induce tremella primordia. Method of opening: Put a piece of tape on the inoculation hole to form a vent hole with a size of a soy bean so as to facilitate oxygen penetration into the material. After 2 days, tear off the tape. You can also tear off the tape on the hole at one time. After opening ventilation, the original tomb of the white mycelium cluster gradually gelatinized to form a crystal-grained ear bud. At this time, the surface of the bag was covered with a newspaper, and moistened with water, the relative humidity of the space was adjusted to 80%, and the temperature was controlled at 18-25°C.

8. Enlargement of the ear; ears 12-13 days after inoculation, when the ear buds are displayed as young ears, the ear is expanded. Operation method: Use a blade to cut off the edge of the film at the edge of the hole to make the oxygen penetrate into the bag better, and accelerate the metabolism and physiological maturity of the mycelium. After expanding the hole, spray the water on the space and bag surface newspaper 2-3 times a day, keep relative humidity 90%-95%, temperature 23-25°C.

9. Moisture styling: 30 days after inoculation, the fruit body grows to a diameter of about 12cm. When it enters mature stage, the water spray is stopped for 8-10 days, the space humidity is reduced to 85%, attention is paid to ventilation, and the ears are stretched and neatly arranged. positive.

10. Harvesting and processing: When the ears are all stretched and flexible, they are cut flat from the base of the ear with a knife, immersed in fresh water, dried in a dewatering machine, and placed on the market.

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