To prevent early buds and early flowering in winter, in order to prevent strawberry early buds and early flowering, the following points should be done in production: First, choose the appropriate variety. Greenhouse cultivation should choose varieties with light dormancy. If you choose a species with strong dormancy, it should not be premature for thermal insulation. The second is to rationally fertilize, strengthen management, and cultivate strong seedlings during the seedling raising process. By removing old leaves, removing inflorescences, straightening stolons, spraying gibberellin and other measures, the management of the mother plant was strengthened to produce high-quality and strong seedlings. The third is to control the temperature after buckling.

It is an effective measure to prevent malformed fruits in the greenhouse by mixing some varieties with high pollen in the main varieties. Pesticides should not be sprayed during strawberry flowering. You can use strawberries to prevent and treat diseases before flowering. After flowering, remove diseased plants in time, remove diseased leaves and inflorescences, and control the spread of the disease. When soil moisture is insufficient, timely mulching with mulch mulch film should be carried out. Combined with watering, 15 kg of 45% ternary compound fertilizer was applied per acre in the absence of fertilizer. Do not apply ammonium bicarbonate. After the fertilizer is diluted with water, the effect of perforating and applying is better. When each batch of strawberry buds in the greenhouse is formed, bees are placed in the shed, and 5,000 are kept in each standard shed, so that the pollination rate can reach 100%. The bee release time is from 8 to 9 am and from 3 to 4 pm.

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DNA/RNA Purification Kit

The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.

Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.

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