The artificial insemination technology of bees plays an important role in the preservation and breeding of the original species and even the engineering breeding. Before artificial insemination, first select the drone, force the sperm, collect semen, select the queen, and then perform artificial insemination. After anesthetizing the queen bee, adjust the microscope and move the back-and-abdom hook control lever under the mirror so that the dorsal and abdomen hooks are under the control of the needle, hooking the central position of the top of the dorsal web respectively, and pulling the dorsal web towards the outside. For 3mm to 4mm, then move the back hook so that most of the hook is embedded in the trigone of the base of the cheek, and then pull the dorsal and abdominal hooks to pull the back-web spacing to 5mm to 6mm. After the needle chamber is opened, adjust the angle between the bee queen and the needle, remove the physiological fluid and air bubbles from the needle tip, and make the needle point close to the vaginal orifice. Use the needle to suppress the flap. Push the needle tip slowly into the vagina until the fallopian tube in the middle and take out the control. The needle slowly injects the semen. One-time insemination generally injects 8 microliters of semen; multiple inseminations each injects 4 microliters to 5 microliters of semen at intervals of 24 hours to 48 hours. After completion of insemination, 10 to 15 seconds later, the needle is withdrawn, the queen bee is removed, and the marker is marked. After recovery, it is sent back to the core group or the box cage for temporary storage. The fertilized bee queen must be strictly managed so that it can be fed by worker bees to ensure that the number of bees in the bee colony of the queen bee in order to regulate the nest temperature. The queen bee can be finned or closed to prevent the queen from going out.